Description
Insulin-Transferrin-Selenium (ITS) Supplement contains Insulin, Transferrin, and Sodium Selenite, prepared in Earle’s Balanced Salt Solution (EBSS) without Phenol Red. In addition, ITS-A and ITS-X contain Sodium Pyruvate and Ethanolamine, respectively. ITS supplementation of many conventional synthetic nutrient media permits substantial reduction in the Fetal Bovine Serum (FBS) requirement for routine maintenance and low-density attachment of many adherent cell types.
Use
• Each 10 mL vial of Insulin-Transferrin-Selenium 100X Supplement is sufficient for up to one liter of medium.
• In general, it is necessary to add 2–4% FBS to achieve optimal growth, although some adherent cultures may require less serum supplementation following initial adaptation.
• Store Insulin-Transferrin-Selenium supplemented medium in the dark at 2°C to 8°C.
Product | Catalog no. | Amount | Storage | Shelf life |
Insulin, Transferrin, Selenium Solution (ITS), 100X | LV-ITS001 | 10ml | 2-8℃ | 12months |
Insulin, Transferrin, Selenium-A Solution (ITS-A), 100X | LV-ITS001A | |||
Insulin, Transferrin, Selenium-X Solution (ITS-X), 100X | LV-ITS001X |
Contents
Components | Concentration (g/L) | ||
ITS | ITS-A | ITS-X | |
Insulin | 1.00 | 1.00 | 1.00 |
Transferrin | 0.55 | 0.55 | 0.55 |
Sodium Selenite | 0.00067 | 0.00067 | 0.00067 |
Sodium Pyruvate | ----- | 11.00 | ----- |
Ethanolamine | ----- | ----- | 0.20 |
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