Transport Mode
Liquid Nitrogen Transportation
1、Adsorptive Liquid Nitrogen Transportation ,No free liquid nitrogen ,White vapor emission indicates normal operation.
2、Temperature monitoring devices should closely monitor the temperature inside the tank during transportation. If any abnormalities are detected, you can copy the PDF and Excel data from the temperature recorder. (One end of the temperature monitoring device can be unplugged to reveal a USB connector. Simply insert it into a computer to copy the data. If you encounter any difficulties, you can contact the sales staff and technical support for assistance.)
3、Alloy Combination Lock Designed to ensure no third-party access to the LN₂ tank or cell samples during transportation from dispatch to receipt, thereby safeguarding cell integrity.
4、GPS Tracker Enables real-time tracking of cell transportation routes to prevent loss.
5、Liquid Nitrogen Tank, temperature monitoring device, alloy combination lock, and GPS tracker are returnable components. Please store them properly and avoid damage; failure to comply will result in blacklisting.
Reference Citation
pass over.
Scope of Application
1、Basic Liver Research Encompassing fundamental studies on liver diseases, viral infections, and gene expression regulation.
2、Drug Development: Encompassing studies on drug efficacy, toxicity, metabolism, and drug-drug interactions.
Gene Manipulation Methods
1. Lentivirus, MOI=30-50 can reach close to 100% infection efficiency, need to test in advance;
2, AAV, MOI=10^5 can reach more than 80% infection efficiency, need to be tested in advance;
3, ASGPR-GalNac free uptake, small nucleic acids need to modify GalNac in advance, can achieve more than 80% delivery efficiency;
4, Lipo2000/3000 is not recommended, high toxicity and low efficiency of primary hepatocyte transfection, unless it has been tested without problems.
Due to biannual updates of our product instruction manual, the following procedures are for reference only.
The most recent version included with the product shall prevail.
II Reagents and Materials
- Primary goose hepatocytes（Cat# LV-PGH001）
- Resuscitation medium（Cat#LV-Rec001）
- Purified medium（If desired, it can be given away with the cells）
- Plating medium（Cat#LV-WEP011）
- Sustaining medium（Cat#LV-WEM011）
- Collagen-coated culture plate（Cat #LV-Coated）
- Sterile centrifuge tube of 15 ml
- Disposable pipette
- Wide-mouth pipette tip (The tip of a normal one is cut off and sterilized)
- Pipette
- Thermostat water bath (Preheat at 38°C)
- Refrigerated horizontal centrifuge (with horizontal rotor. it is possible to centrifuge 15 ml of centrifuge tubes)
- Biosafety cabinet
- 37 °C/5% CO2 Incubator
- 75%Alcohol
IV Maintenance and Culture of Hepatocyte
1. Culture for 16 h or overnight can make the cells adherent. Then remove the plating medium (including some dead cells), add sustaining medium, and change the solution every 2 days.
2. Primary goose hepatocytes have limited proliferative capacity with obvious contact inhibition properties. The differentiation characteristics and the incubation time of hepatocytes depend on high-density of cell culture.
Primary goose hepatocytes maintain relatively short time in vitro. It is recommended that the experiment be completed within 3 days after plating, and the maximum is not more than 5 days. The subculture of goose primary hepatocytes is not recommended.
Post-Thaw Cells Must Not Undergo Refreezing.
V Customer Service
If you find any quality problems with the product, please collect the original data and contact the company's salesmen or technical support at the first time. The company ensure after-sales service. Every laboratory has different conditions, different operating habits, different proficiency, and objective factors in experimental failure. If the operation is not strictly in accordance with the instructions or exceeds the time limit of after-sales, the company does not do after-sales. Please understand and support us.
Validity period and raw data provided:
Resuscitation problems: Within 24 hours of resuscitation, trypan blue staining or PI staining should be provided.
Pollution problems: Within 96 hours of resuscitation, microscope photographs of differences should be provided.
Purity issues: Within one month, immunofluorescence or flow cytometry results should be provided
VI Contact Number
Tel:0755-28284050
Technical Support: 19902901483 (Dr. Zhou)
